Selectively cleavabe linners based on iminodiacetic acid esters for solid phase peptide synthesis
US5635598A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 21, 1994 |
| Grant date | Jun 3, 1997 |
| Priority date | — |
| Expiry date | Jun 21, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/042
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to linkers based on ester bond linkages, especially iminodiacetic acid ester bond linkages, for use in solid phase peptide synthesis. In particular, the invention is directed to cleavable linkers that can release peptide from the solid phase support under relatively mild conditions by formation of a diketopiperazine or other cyclic structure, such that the cyclic structure remains on the solid phase support, and, in a second cleavage, under more stringent conditions of high pH. The invention is further directed to solid phase supports prepared with multiple cleavable linkers, including a linker that is cleaved by formation of a cyclic product. One such second linker is an ester of hydroxymethylbenzoic acid, or esters formed by carboxy groups of aspartic or glutamic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.