Design and synthesis of bispecific DNA-antibody conjugates
US5635602A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 13, 1993 |
| Grant date | Jun 3, 1997 |
| Priority date | — |
| Expiry date | Aug 13, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/468
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to bis-protein-DNA conjugates. A protein having an antigen specific binding activity is covalently linked to each end of a derivatized DNA molecule. The bis-protein-DNA conjugates can be used for immunoassays and measuring distances between proteins at up to 3.4 .ANG. resolution. The invention also relates to methods of synthesizing these bis-protein-DNA conjugates. Synthesis of the conjugates entails derivatizing the 5' or 3' end of a DNA oligonucleotide and covalently linking that DNA to a protein. The DNA can be indirectly conjugated to an antibody or Fab' fragment, using a avidin/streptavidin-biotin linkage. The conjugates of the invention can be used in immunoassays and PCR assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.