Recombinant protein production and insect cell culture and process

US5637477A · kind A · utility

Assignee

• The United States of America as represented by the Administrator of the National Aeronautics and Space Administration · US

Inventors

• Glenn F. Spaulding · Houston, US
• Thomas J. Goodwin · Friendswood, US
• Kim C. O'Connor · New Orleans, US
• Karen M. Francis · Aiken, US
• Angela D. Andrews · Pensacola, US
• Tacey L. Prewett · Friendswood, US

Key dates

• Filing date: Aug 16, 1994
• Grant date: Jun 10, 1997
• Expiry date: Aug 16, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2710/14143
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process has been developed for recombinant production of selected polypeptides using transformed insect cells cultured in a horizontally rotating culture vessel modulated to create low shear conditions. A metabolically transformed insect cell line is produced using the culture procedure regardless of genetic transformation. The recombinant polypeptide can be produced by an alternative process using virully infected or stably transformed insect cells containing a gene encoding the described polypeptide. The insect cells can also be a host for viral production.