Dextranase enzyme, method for its production and DNA encoding the enzyme
US5637491A · kind A · utility
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Key dates
| Filing date | Dec 13, 1994 |
| Grant date | Jun 10, 1997 |
| Priority date | — |
| Expiry date | Dec 13, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/2454
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungus was isolated and sequenced. This cDNA was transfered into Pichia pastoris cells. Recombinant yeasts capable of secreting dextranase to the culture medium were obtained thereby. The dextranase enzyme obtained can be used, e.g. in the sugar industry to hydrolyze the dextran in cane juices to increase the sugar production.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.