Modulated homogeneous fluorescence biospecific affinity assay employing fluorescing lanthanide chelates as covalent labels
US5637509A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 12, 1990 |
| Grant date | Jun 10, 1997 |
| Priority date | — |
| Expiry date | Dec 12, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/826
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method for a homogeneous biospecific affinity assay for determining the content of a substance (analyte) in a biological sample. The assay is carried out in an aqueous reaction medium by means of time-resolved fluorescence spectroscopy and with a biospecific affinity reactant labeled with a lanthanide chelate in which a lanthanide ion exhibiting ionic fluorescence is chelated by a ligand bound covalently to the reactant. The characteristic feature is PA1 (i) that the lanthanide chelate formed by the lanthanide ion together with the covalently bound ligand forms a fluorescent chelate, and PA1 (ii) that a modulator is added which stabilizes the lanthanide chelate so that the lanthanide fluorescence as measured from the medium becomes a practically pure function of the analyte concentration therein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.