Patent · US Expired

DNA construct and in vitro test for detecting tumor promoters by means of said DNA construct

US5639596A · kind A · utility

7Cited by

0References

13Claims

0Family size

Assignee

GSF FORSCHUNGSZENTRUM FUR UMWELT UND GESUNDHEIT GMBH · DE

Inventors

Georg Bornkamm · Hersbruck, DE
Axel Polack · Hersbruck, DE

Key dates

Filing date	Jul 25, 1994
Grant date	Jun 17, 1997
Priority date	—
Expiry date	Jul 25, 2014

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2800/108
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a DNA construct and to a method of detecting tumor promoters by employing this DNA construct. It is an object of the invention to make available an easily implemented quantitative detection method for tumor promoters. This is accomplished by a DNA construct including at least one reporter gene and a control region for the activation of a particularly strongly induced gene of the Epstein-Barr virus (EBV), and by the action of the substance to be examined on EBV-containing cells that contain the DNA construct, whereupon the resulting quantity of reporter gene product is quantitatively determined.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.