Amplification of nucleic acids from mononuclear cells using iron complexing and other agents
US5639599A · kind A · utility
60Cited by
7References
129Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 6, 1994 |
| Grant date | Jun 17, 1997 |
| Priority date | — |
| Expiry date | Jun 6, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Method and kit for the preparation of nucleic acid, such as for amplification. The nucleic acid may be prepared by centrifuging a sample of whole cells, particularly mononuclear cells, with an appropriate centrifugation medium so as to separate desired cells from non-desired cells, then removing and lysing said desired cells. A complexing agent able to complex ferric ion may be added to the lysed cells. The nucleic acid associated with the lysed cells can be amplified, and the nucleic acid can also be screened for a certain nucleic acid sequence, such as a vital nucleic acid sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.