Machine for synthesizing oligonucleotides
US5641459A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 17, 1995 |
| Grant date | Jun 24, 1997 |
| Priority date | — |
| Expiry date | Aug 17, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B60/14
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A machine for synthesizing oligonucleotides has individual pumping modules for connection to each of a number of different monomers and to other fluids used in the synthesizing process. Each pumping module includes a pump and a three-port/three-way valve with the first inlet port connected to receive the fluid, and the second port connected to receiving a flushing agent, such as acetonitrile. A valving arrangement downstream from the pumping modules selects a monomer. The selected monomer and other fluids pass through flow sensors which provides signals to a controller, which uses these signals to regulate the pumping of the pumps. At least some of the valves allow multiple inlet ports to be kept open at the same time, thus allowing certain of the liquids to be mixed within the valves. The various pumps, valves and sensors are mounted to the exterior of a cabinet and arranged such that the liquid handling portions are exterior to the cabinet, while electrical portions are within the cabinet. Air is continuously purged within the cabinet in order to prevent the introduction of solvent fumes near electrical components. The machine can produce up to 100 millimoles of oligonucleotides,…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.