Fluorescence polarization detection of nucleic acids
US5641633A · kind A · utility
30Cited by
4References
26Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 15, 1995 |
| Grant date | Jun 24, 1997 |
| Priority date | — |
| Expiry date | Nov 15, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods for detecting amplified or unamplified nucleic acid target sequences at increased temperatures by changes in fluorescence polarization. The decrease in fluorescence polarization associated with hybridization of oligonucleotides at higher, more stringent, temperatures is overcome by including a double-stranded DNA binding protein in the assay. At elevated temperatures, the double-stranded DNA binding protein restores, and often enhances, the magnitude of the change in fluorescence polarization associated with single- to double-stranded conversion of an oligonucleotide probe or primer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.