Synthetic herpes simplex virus promoters
US5641651A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Oct 31, 1994 |
| Grant date | Jun 24, 1997 |
| Priority date | — |
| Expiry date | Oct 31, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to synthetic herpes simplex virus (HSV) promoters which are constructed by operatively linking the 5' nontranscribed domain of an HSV .alpha. gene to a fragment containing the transcription initiation site and the 5' transcribed noncoding region from an HSV .gamma. gene. Synthetic promoters of the invention that are operatively linked to heterologous genes, inserted into HSV genomes and used to generate live virus are useful for expressing polypeptides encoded by the heterologous genes in appropriate host cells. The synthetic promoters direct transcription of the heterologous genes constitutively throughout the reproductive cycle of the virus at a high cumulative level. The recombinant viruses of the invention can also be used as vaccines to present polypeptides against which a host will mount an immune response.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.