Process for transforming monocotyledonous plants

US5641664A · kind A · utility

Assignee

• Plant Genetic Systems N.V. · BE

Inventors

• Kathleen D'Halluin · Mariakerke, BE
• Elke Gobel · Gent, BE

Key dates

• Filing date: Jun 23, 1993
• Grant date: Jun 24, 1997
• Expiry date: Jun 23, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8289
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A rapid and efficient method for transforming monocotyledonous plants, especially gramineous plants, particularly corn, using either intact tissue capable of forming compact embryogenic callus or compact embryogenic callus obtained from such tissue to obtain transgenic monocotyledonous plants, is provided. Preferably, the tissue or embryogenic callus is wounded and/or degraded prior to transformation. The resulting wounded and/or degraded, intact tissue or compact embryogenic callus is then transformed, preferably by direct gene transfer, e.g., by means of electroporation, with one or more DNA fragments (e.g., foreign DNA fragments).