Methods for protein binding enzyme complementation assays
US5643734A · kind A · utility
25Cited by
1References
6Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jun 1, 1995 |
| Grant date | Jul 1, 1997 |
| Priority date | — |
| Expiry date | Jun 1, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/807
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.