Transposon-containing DNA cloning vector and uses thereof
US5645991A · kind A · utility
11Cited by
2References
19Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | Mar 14, 1995 |
| Grant date | Jul 8, 1997 |
| Priority date | — |
| Expiry date | Mar 14, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.