Hybrid vector and method resulting in protein overproduction by eukaryotic cells
US5646009A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 19, 1994 |
| Grant date | Jul 8, 1997 |
| Priority date | — |
| Expiry date | Jan 19, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/67
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A hybrid vector carrying a first and second DNA segments operationally linked thereto, the first DNA segment encoding a protein capable of cross-linking to the cap structure of mRNA and mediating ribosome-binding, and the second DNA segment encoding a polypeptide or protein, the vector being capable of replication, transcription and translation to express the factor and the polypeptide or protein upon transformation of a eukaryotic host, and the polypeptide or protein being expressed at a level higher than the level of expression thereof in the absence of the first DNA segment. A eukaryotic host is transformed with this hybrid vector. Also disclosed is a method of increasing the synthesis of a polypeptide or protein in a eukaryotic host cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.