Plant arabinogalactan protein (AGP) genes

US5646029A · kind A · utility

Assignee

• Cooperative Research Centre For Industrial Plant Biopolymers · AU

Inventors

• Chao-Guang Chen · Melbourne, AU
• Shaio-Lim Mau · Melbourne, AU
• He Du · Sunnyvale, US
• Alison M. Gane · Melbourne, AU
• Antony Bacic · Melbourne, AU
• Adrienne Elizabeth Clarke · Melbourne, AU

Key dates

• Filing date: Jul 18, 1994
• Grant date: Jul 8, 1997
• Expiry date: Jul 18, 2014

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention provides plant arabinogalactan proteins (AGPs) and their genes. AGPs were isolated from Nicotiana alata, Nicotiana plumbaginafolia, and Pyrus communis. Amino acid sequences of isolated AGP peptide fragments are presented. Isolated AGP fragments were used to synthesize oligonucleotide probes to prepare oligonucleotide primers for PCR or prepare RNA probes to screen cDNA libraries of N. alata, N. plumbaginafolia, and P. communis. cDNA clones encoding amino acid sequences of isolated AGP fragments were isolated. The invention presents for the first time an intact AGP amino acid sequence derived from a corresponding AGP gene. The instant invention further provides methods useful in obtaining AGP genes encoding an AGP peptide comprising a specific isolated hydroxyproline-rich (OAST-rich) sequence or a specific isolated hydroxyproline-poor sequence.