Method for isolating mutant cells
US5646030A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 23, 1994 |
| Grant date | Jul 8, 1997 |
| Priority date | — |
| Expiry date | Aug 23, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2795/10351
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for isolating a mutant cell that excretes a desired compound. The method includes culturing a plurality of auxotrophic pretreated starter cells and auxotrophic feeder cells in the presence of a reversibly noninfective, modified lambdoid bacteriophage. If the treated starter cell produces the desired compound, the bacteriophage will be rendered infective and infect the feeder cell. The feeder cell, in turn, will excrete a metabolite required by the starter cell and the starter cell will excrete a metabolite required by the feeder cell, enabling the cells to cross-feed, grow, and produce a colony containing a starter cell which produces the desired compound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.