High efficiency method for isolating target substances using a multisample separation device

US5646263A · kind A · utility

Assignees

• Promega Corporation · US
• THE JOHNS HOPKINS UNIVERSITY · US

Inventors

• Steven J. Ekenberg · Verona, US
• Paula R. Brisco · Oregon, US
• Anne Louise Oaklander · Baltimore, US

Key dates

• Filing date: Sep 19, 1994
• Grant date: Jul 8, 1997
• Expiry date: Sep 19, 2014

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2035/00495
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods for separating target particles, from a test medium in which the particles are suspended. The method contemplates utilization of blocking or masking particles which block or mask unwanted biological debris which tends to interfere with formation of complexes by which the target particles can be isolated. After centrifugation, the masking particles create a barrier between unwanted debris and test medium containing the target particles which permits the particles to be manipulated or modified without removing them from the debris. In a preferred aspect, magnetic separation techniques are used. In this aspect, non-magnetic 0.04 micron carboxylated polystyrene blocking particles are employed.