Patent · US Expired

Automatic focusing of biomedical specimens apparatus

US5647025A · kind A · utility

111Cited by
18References
30Claims
0Family size

Assignee

Inventors

Key dates

Filing dateSep 20, 1994
Grant dateJul 8, 1997
Priority date
Expiry dateSep 20, 2014

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG02B7/28
  • WIPO fieldOptics
  • WIPO sectorInstruments

Abstract

A method for automatically focusing on a slide includes the steps of locating a coverslip, acquiring images from predetermined focal depths in the slide, and starting at an initial focal depth proximate the surface of the coverslip. A set of predetermined characteristics within each of the images are measured to generate at least one image measurement for each of the plurality of images. A focus measure is computed for each of the images, where each focus measure is a function of at least one image measurement. A best focus location is determined relative to a focal depth where an acquired image has a highest focus measure.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.