Microbiological best method and reagents
US5648232A · kind A · utility
38Cited by
3References
45Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Apr 18, 1996 |
| Grant date | Jul 15, 1997 |
| Priority date | — |
| Expiry date | Apr 18, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2304/60
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for detecting the presence and/or mount of microorganisms is described by adding adenosine diphosphate (ADP) to a sample suspected of containing microorganisms and/or their intracellular material, determining the amount of adenosine triphosphate (ATP) generated by adenylate kinase present, for example using a bioluminescent assay involving luciferase and luciferin, and relating the results to the presence and/or amount of microorganism. Test kits and apparatus for use in the method are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.