Production, purification, cleavage and use of fusion peptides
US5648244A · kind A · utility
28Cited by
2References
44Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 27, 1993 |
| Grant date | Jul 15, 1997 |
| Priority date | — |
| Expiry date | Sep 27, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for producing a fusion peptide. A vector is provided with nucleic acid encoding a carrier peptide and at the 3' end of the nucleic acid, a unidirectional restriction endonuclease cleavage site recognized by a restriction endonuclease with the ability to create a non-palindromic 3-base overhang. The vector is cleaved with the restriction endonuclease to produce a cleaved vector. One or more nucleic acids encoding a desired peptide and having at least a 3-base overhang at each end configured and arranged for ligation with the cleaved vector is then ligated to the cleavage site.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.