Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5648245A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 9, 1995 |
| Grant date | Jul 15, 1997 |
| Priority date | — |
| Expiry date | May 9, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An in vitro method for constructing an oligonucleotide concatamer library is revealed. The method has four steps. The first step involves the generation of a primer-bridged circular template oligonucleotide. In the second step, the primer-bridged circular template is used to generate a single-stranded oligonucleotide concatamer by rolling circle replication. In the third step, the single-stranded oligonucleotide concatamer is converted to a double-stranded oligonucleotide concatamer. Finally, the double-stranded oligonucleotide concatamer is cloned or used directly in in vitro assays which allow molecules of interest to be isolated or amplified.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.