Method of transduction of cells with retroviral vectors
US5648251A · kind A · utility
3Cited by
3References
10Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 26, 1995 |
| Grant date | Jul 15, 1997 |
| Priority date | — |
| Expiry date | May 26, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/10051
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to an efficient method, termed spin transduction, of transducing cells with retroviral vectors. Retroviral particles, suspended in a pharmaceutically acceptable carrier, are contacted with the cells to be transduced, and the mixture is centrifuged for a sufficient period of time to permit transduction. This method leads to a 3- to 15-fold increase in transduction efficiency, depending on the type of cells transduced.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.