Enzymatic synthesis of oligonucleotides
US5650271A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 1, 1993 |
| Grant date | Jul 22, 1997 |
| Priority date | — |
| Expiry date | Apr 1, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/582
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention makes possible the catalytic production of sequence specific oligonucleotides through the use of a specially designed template sequence. The reaction can be made to proceed isothermally in the presence of an excess of nucleoside triphosphates, an agent for polymerization, and a cutting agent. Because the process is catalytic with respect to the template sequence, an unlimited amount of oligonucleotide product can theoretically be generated from a single molecule of template. Where the process is initiated by the presence of a nucleic acid target sequence, the method of the present invention can be used for diagnostic purposes as an amplification method to improve sensitivity. Diagnostic sensitivity can be further enhanced by employing a cascade of these template sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.