Process and culture medium for the production of cells infected by a multiple sclerosis-associated virus
US5650318A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 2, 1994 |
| Grant date | Jul 22, 1997 |
| Priority date | — |
| Expiry date | Feb 2, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2469/20
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Process for in vitro production of a culture or cell line infected by a viral strain associated with multiple sclerosis (MS), according to which a body sample is taken from an individual suffering from MS, said sample is cultivated in a culture medium which promotes the growth of infected cells to obtain a culture of primary infected cells, and a sample of the culture of primary cells or of a subculture of the latter is cultivated in series, that is to say by successive passages, in said culture medium to obtain the culture or cell line infected by a virus associated with MS, which comprises a procedure in which the culture medium also contains a beta anti-interferon antibody or an antibody which is directed against an antigenically close molecule, the antibody playing an inhibiting role in viral expression and allowing long-lasting expression and propagation of the viral strain in the culture or cell line.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.