Identification of allele specific nucleic acid sequences by hybridization with crosslinkable oligonucleotide probes
US5652096A · kind A · utility
38Cited by
6References
14Claims
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Key dates
| Filing date | Apr 20, 1994 |
| Grant date | Jul 29, 1997 |
| Priority date | — |
| Expiry date | Apr 20, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/82
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Hybridization method for discriminating between complementarity and partial complementarity of DNA base sequences. Kinetic covalent entrapment of PCR-amplified target DNA is achieved through the use of crosslinkable probes. A crosslinking site is introduced into the target DNA via the PCR amplification process. Problems with renaturation of target DNA in the PCR process and in the hybridization reaction are minimized.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.