In vitro test to evaluate the inhibition potency of angiotensin II antagonists on angiotensinogen
US5652097A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 14, 1994 |
| Grant date | Jul 29, 1997 |
| Priority date | — |
| Expiry date | Dec 14, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to an in vitro test by recombinant DNA technology for the evaluation of the potency of various angiotensin II (AII) antagonists on the inhibition of expression of angiotensinogen (ANG) gene in vivo. A transformed human liver cell line capable of expressing human angiotensinogen-growth hormone, which includes at least one DNA construct coding for at least a 5'-flanking region of the ANG operatively linked upstream to human growth hormone gene. An in vitro test for the evaluation of the potency of various angiotensin II (AII) antagonists on the inhibition of expression of angiotensinogen (ANG) gene in vivo, which comprises administering AII antagonists to a transformed human liver cell line of claim 1 and monitoring the expression of human angiotensinogen-growth hormone.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.