RNA amplification method requiring only one manipulation step
US5654143A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 27, 1995 |
| Grant date | Aug 5, 1997 |
| Priority date | — |
| Expiry date | Mar 27, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An RNA amplification method is particularly useful for diagnosing bacterial or viral infections or genetic disorders and for cell typing. The method includes the steps of denaturing a solution containing RNA, synthesizing a first cDNA strand from a suitable primer in the presence of reverse transcriptase, denaturing the heteroduplex formed, synthesizing a second cDNA strand from a second primer in the presence of DNA polymerase and then subjecting the cDNA formed to a sufficient number of amplification cycles. All the reactants and solvents are first placed in the same container to provide a single manipulation step that avoids the risk of contamination.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.