Nucleic acid preparation methods
US5654179A · kind A · utility
40Cited by
24References
16Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Oct 3, 1994 |
| Grant date | Aug 5, 1997 |
| Priority date | — |
| Expiry date | Oct 3, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25375
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides an improved method for the preparation of ribonucleic acid (RNA) samples. This method utilizes heat and guanidinium thiocyanate treatment of samples followed by alcohol precipitation and centrifugation to prepare RNA samples with a high degree of sensitivity, reliability, and ease of use. Importantly, the prevent invention provides a method in which RNA samples may be prepared so as to conserve RNA preservation and precipitation reagents and time. The samples so prepared are readily amplifiable and may be used for other purposes as well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.