Cyclized fluorescent nucleic acid intercalating cyanine dyes and nucleic acid detection methods
US5658735A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 9, 1995 |
| Grant date | Aug 19, 1997 |
| Priority date | — |
| Expiry date | Nov 9, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
New intercalating cyanine dyes are provided in which the benzothiazole portion of the cyanine dye has been modified to produce dyes with improved properties for labelling nucleic acids. These new intercalating cyanine dyes are cyclized fluorescent cyanine dyes represented by General Formula I ##STR1## where: n is 0, 1 or 2; PA1 Y may be either S or O; PA1 R.sub.3 and R.sub.4 may each independently be either hydrogen, C.sub.1 -C.sub.10 alkyl, C.sub.1 -C.sub.10 alkoxy or C.sub.1 -C.sub.10 alkylthio; PA1 R.sub.5 may be C.sub.1 -C.sub.50 alkyl, preferably substituted with one or more polar substituents which preferably includes one or more positively charged atoms, or a cyclized fluorescent cyanine dye of the present invention, i.e., where R.sub.5 is a linker between two cyclized fluorescent cyanine dyes; PA1 R.sub.6 and R.sub.7 may each independently be either H and C.sub.1-10 alkyl, or may be taken together to form a 5 or 6 membered ring, most preferably a 6 membered aromatic ring, optionally substituted with C.sub.1-6 alkyl or C.sub.1 -C.sub.10 alkoxy groups; PA1 R.sub.8 and R.sub.9 may each independently be either H and C.sub.1-10 alkyl, or may be taken together to form a 5 or 6 me…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.