Cell-free synthesis and isolation of novel genes and polypeptides
US5658754A · kind A · utility
138Cited by
4References
50Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Nov 29, 1991 |
| Grant date | Aug 19, 1997 |
| Priority date | — |
| Expiry date | Nov 29, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6867
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the cell-free synthesis and isolation of novel genes and polypeptides is provided. Within one embodiment, an expression unit is constructed onto which semi-random nucleotide sequences are attached. The semi-random nucleotide sequences are first transcribed to produce RNA, and then translated under conditions such that polysomes are produced. Polysomes which bind to a substance of interest are then isolated and disrupted; and the released mRNA is recovered. The mRNA is used to construct cDNA which is expressed to produce novel polypeptides.
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