Selection of diagnostic genetic markers in microorganisms and use of a specific marker for detection of salmonella
US5660981A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 6, 1994 |
| Grant date | Aug 26, 1997 |
| Priority date | — |
| Expiry date | Jun 6, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is provided for the selection of diagnostic genetic markers fragments and useful in the identification of bacteria at the genus, species or serotype level. The method first involves the identification of a RAPD polymorphic DNA fragment common to a particular microbial group, the identification of the most conserved regions of that fragment, and the preparation of specific primers useful for detecting the presence of a marker within the fragment whereby that set of primers is then useful in the identification of all members of the chosen microbial group. Also provided is a specific diagnostic marker for Salmonella and primers directed thereto.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.