Recombinant human factor VIII derivatives

US5661008A · kind A · utility

Assignee

• Kabi Pharmacia AB · SE

Inventors

• Annelie Almstedt · Stockholm, SE
• Eva Maria Gray (Hellstrom) · Stockholm, SE
• Peter Lind · Huddinge, SE
• Catherine Ljung · Sundbyberg, SE
• Helena Sandberg · Vingåker, SE
• Jack Spira · Tyresö, SE
• Mona Sydow-Backman · Saltsjöbaden, SE
• Helena Wiman · Stockholm, SE

Key dates

• Filing date: Jun 5, 1995
• Grant date: Aug 26, 1997
• Expiry date: Jun 5, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/755
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A DNA sequence coding for a biologically active recombinant human factor VIII derivative, comprising a first DNA segment coding for the amino acids 1 through 740 of human factor VIII and a second DNA segment coding for the amino acids 1649 through 2332 of human factor VIII, said segments being interconnected by a linker DNA segment coding for a linker peptide of at least 3 amino acid residues and up to about 10 amino acid residues which are selected from lysine and arginine; recombinant expression vector comprising such DNA sequence; host cells of animal origin transformed with such recombinant expression vector; a process for the manufacture of recombinant human factor VIII derivative; and human factor VIII derivative containing the heavy chain and the light chain linked by metal ion bond.