Recombinant production of consensus human leukocyte interferon
US5661009A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 26, 1996 |
| Grant date | Aug 26, 1997 |
| Priority date | — |
| Expiry date | Jul 26, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/142
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Described are rapid and highly efficient procedures for the total synthesis of linear, double stranded DNA sequences in excess of about 200 base pairs in length, which sequences may comprise entire structural genes. Novel sequences are prepared from two or more DNA subunits provided with terminal regions comprising restriction endonuclease cleavage sites facilitating insertion of subunits into a selected vector for purposes of amplification during the course of the total assembly process. The total, finally-assembled sequences include at least one, and preferably two or more, unique restriction endonuclease cleavage site(s) at intermediate positions along the sequence, allowing for easy excision and replacement of subunits and the correspondingly facile preparation of multiple structural analogs of polypeptides coded for by the sequences. Manufactured genes preferably include codons selected from among alternative codons specifying the same amino acid on the basis of preferential expression in a projected host microorganism (e.g., E. coli) to be transformed. Illustrated is the preparation and expression of manufactured genes capable of directing synthesis of human immune and leukoc…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.