Production and purification of retroviral particles using tentacle anion exchange
US5661023A · kind A · utility
6Cited by
5References
10Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 11, 1996 |
| Grant date | Aug 26, 1997 |
| Priority date | — |
| Expiry date | Mar 11, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16122
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention is directed to a continuous process for producing viral particles by providing in a perfused growth medium a population of viable virally infected non-lytic cells, and removing medium containing said cells at a rate to maintain the steady-state log-phase growth of cells remaining in the perfused growth medium. The invention is also directed to a process for purifying retroviral particles by passing a solution comprising the retroviral particles and contaminants through an anion exchange resin, and eluting the retroviral particles from the resin. The preferred exchange resin is a tentacle anion exchange resin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.