Benzodiazepine protein conjugates

US5662911A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Erasmus Huber · Garching bei München, DE
• Christian Klein · Iffeldorf, DE
• Hans-Peter Josel · Weilheim, DE
• Bruno Zink · Murnau am Staffelsee, DE

Key dates

• Filing date: Jan 24, 1996
• Grant date: Sep 2, 1997
• Expiry date: Jan 24, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K16/44
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

Benzodiazepine protein conjugates of the formula I ##STR1## wherein R1 is hydrogen, a methyl group or R, R2 is hydrogen, a hydroxyl or an OR group, with R1 or R2 containing an R group, R3 is a halogen, NO2 or NH2, X is hydrogen or a halogen, wherein R is a group of the formula II, wherein Z is a macromolecular immunogen reactive carrier substance and n is 2 or 3. The benzodiazepine protein conjugates are used as immunogens to obtain antibodies to benzodiazepines. The invention also relates to an immunoassay for the detection of benzodiazepines where the so generated antibodies are used.