Immuno-polymerase chain reaction system for antigen detection
US5665539A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 4, 1993 |
| Grant date | Sep 9, 1997 |
| Priority date | — |
| Expiry date | Oct 4, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2458/10
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A novel system and method for sensitive antigen detection. The system utilizes immuno-polymerase chain reaction in which a specific biotinylated nucleic acid molecule is used as the marker. The biotinylated marker is attached to antigen-antibody complex through a streptavidin-protein A chimeric protein that possesses tight and specific binding affinity both for biotin and immunoglobulin G. A segment of the attached biotinylated marker is amplified by polymerase chain reactions with appropriate primers and the polymerase chain reaction products are detected by agarose gel electrophoresis. The method can detect any antigen and has a greater sensitivity than any existing antigen detection system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.