Preparation of heterodimeric PDGF-AB using a bicistronic vector system in mammalian cells

US5665567A · kind A · utility

Assignees

• BEIERSDORF AG · DE
• Gesellschaft fur Biotechnologische Forschung mbH (GBF) · DE

Inventors

• Wolfram Eichner · Rockenberg, DE
• Volker Achterberg · Hamburg, DE
• Albrecht Dorschner · Hamburg, DE
• Wolfgang Meyer-Ingold · Hamburg, DE
• Heiko Mielke · Neu Wulmstorf, DE
• Wilhem Dirks · Braunschweig, DE
• Manfred Wirth · Leipzig, DE
• Hansjorg Hauser · Braunschweig, DE

Key dates

• Filing date: Apr 24, 1995
• Grant date: Sep 9, 1997
• Expiry date: Apr 24, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/44
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The recombinant production of PDGF-AB in mammalian cells is disclosed by means of a bicistronic vector system in which an IRES sequence is located between the first and second cistrons and in which the B chain coding gene is located in the first cistron. The disclosed expression unit allows the equimolor expression of the A and B polypeptide chains.