Yeast promoter for expressing heterologous polypeptides
US5667986A · kind A · utility
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1References
12Claims
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Key dates
| Filing date | Jul 1, 1994 |
| Grant date | Sep 16, 1997 |
| Priority date | — |
| Expiry date | Jul 1, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A yeast promoter comprising the promoter of the (cytoplasmically located glycerol-3-phosphate dehydrogenase gene (GPD 1) possesses advantageous capacity to regulate the expression of heterologous polypeptides in yeasts transformed therewith. Expression of such polypeptides utilizing the GPD1 promoter can be regulated by the presence(repressed) or absence(derepressed) of high levels of sucrose or glucose in the fermentation medium. Alternatively, a non-repressing carbon source, such as glycerol or ethanol, can be added to the fermentation medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.