Hybrid molecules having translocation region and cell-binding region
US5668255A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 4, 1993 |
| Grant date | Sep 16, 1997 |
| Priority date | — |
| Expiry date | Aug 4, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2770/24122
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A hybrid molecule including a first part and a second part connected by a covalent bond, PA1 (a) the first part including a portion of the binding domain of a cell-binding ligand, which portion is able to cause the hybrid molecule of the invention to bind to an animal cell; and PA1 (b) the second part including a portion of a translocation domain of a protein, provided that (i) the hybrid molecule does not include an enzymatically-active portion of the protein, (ii) the first part and the second part are not segments of the same naturally-occurring polypeptide toxin, and (iii) the portion of the translocation domain, when covalently bonded to the enzymatically-active effector region of a toxin selected from diphtheria toxin, Pseudomonas exotoxin A, cholera toxin, ricin toxin, and Shiga-like toxin, is capable of translocating such effector region across the cytoplasmic membrane of the cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.