Method for producing synthetic N-linked glycoconjugates
US5668272A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 30, 1995 |
| Grant date | Sep 16, 1997 |
| Priority date | — |
| Expiry date | Jun 30, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/685
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A transamination reaction of glycosyl-1-amine with a nucleophilic reagent containing an --NH.sub.2 or --NHNH.sub.2 group is used to prepared a broad spectrum of glycoconjugates by substitution of the 1-amino group. The substitution reaction does not adversely affect the ring structure of the sugar as does the prior art reductive amination reaction. Suitable nucleophilic reagents are nucleophiles having a chromophore group, a fluorophore group, a chemiluminescent group, a lipid, an amino acid or peptide moiety, biotin, a drug and a linker/spacer group and an affinity label. The ratio of glycosyl-1-amine to nucleophilic reagent is in the range of from about 1:1 to about 1:2 by weight.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.