Nucleotide-containing sorbent for affinity chromatography

US5670049A · kind A · utility

Assignee

• Merck Patent GmbH · DE

Inventors

• Egbert Muller · Langen (Hessen), DE
• Jurgen Hemberger · Aschaffenburg, DE
• Michael Morr · Wolfenbüttel, DE

Key dates

• Filing date: Apr 1, 1996
• Grant date: Sep 23, 1997
• Expiry date: Apr 1, 2016

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N30/02
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to sorbents for for sic! affinity chromatography which are based on hydroxyl-containing base supports on whose surfaces polymers are covalently bonded, characterized in that PA0 a) the base support contains aliphatic hydroxyl groups, PA0 b) the covalently bonded polymers are bound to the base support by a terminal monomer unit, PA0 c) the polymers contain monomer units of the formula II PA0 d) the monomer units are linked linearly, ##STR1## in which R.sup.1, R.sup.2 and R.sup.3 independently of one another are H or CH.sub.3, PA1 R.sup.4 is H, C.sub.1 -C.sub.5 -alkyl or C.sub.6 -C.sub.12 -aryl, PA1 n is an integer between 1 and 5 and one of the radicals X is OH and the other radical X is a nucleoside-containing radical, and to their preparation and use, especially for affinity chromatography.