Cryoprotective aqueous solutions useful for the preservation of in vitro cultured epithelial sheets
US5670308A · kind A · utility
3Cited by
8References
6Claims
0Family size
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Key dates
| Filing date | Jun 1, 1995 |
| Grant date | Sep 23, 1997 |
| Priority date | — |
| Expiry date | Jun 1, 2015 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01N1/10
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
A method for the preservation of in vitro expanded epithelial sheets by incubation of the sheets in a cryopreserving solution characterized by lack of volume increase at the liquid-solid phase transition. The cryopreserving solutions comprise: a) polyethyleneglycols having molecular weight between 3 and 5 KD; and b) at least one cross-linking agent selected from polyols, polyamines, mono- or oligosaccharides, and polyethyleneglycols having molecular weight lower than 1 KD.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.