Diagnostic applications of double D-loop formation
US5670316A · kind A · utility
74Cited by
6References
21Claims
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Key dates
| Filing date | May 25, 1994 |
| Grant date | Sep 23, 1997 |
| Priority date | — |
| Expiry date | May 25, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/683
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention describes the formation of RecA protein catalyzed double-stranded probe:duplex linear target DNA complexes that are stable to deproteinization. The uses of this stable probe:target complex in diagnostic/DNA detection systems in in vitro and in situ DNA hybridization reactions is discussed. The probe:target complexes are also useful for diagnostic application in RecA protein facilitated DNA amplification reactions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.