Methods and compositions for the ex vivo replication of human hematopoietic stem cells

US5670351A · kind A · utility

Assignee

• THE REGENTS OF THE UNIVERSITY OF MICHIGAN · US

Inventors

• Stephen G. Emerson · Ann Arbor, US
• Michael F. Clarke · Ann Arbor, US
• Bernhard O. Palsson · Village of La Jolla, US
• Richard Schwartz · Ann Arbor, US

Key dates

• Filing date: Dec 30, 1994
• Grant date: Sep 23, 1997
• Expiry date: Dec 30, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/85
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods, including culture media conditions, which provide for ex vivo human stem cell division and stable genetic transformation and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.