Patent · US Expired

Recombinant fowlpox virus

US5670367A · kind A · utility

23Cited by

4References

9Claims

0Family size

Assignee

Immuno Aktiengesellschaft · AT

Inventors

Friedrich Dorner · Wien, AT
Friedrich Scheiflinger · Wien, AT
Falko-Günter Falkner · Orth an der Donau, AT

Key dates

Filing date	Apr 22, 1994
Grant date	Sep 23, 1997
Priority date	—
Expiry date	Apr 22, 2014

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2710/24043
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

An improved method is described to prepare recombinant fowlpox virus for the expression of proteins or for use as a vaccine. The new method uses for the insertion of foreign DNA an intergenic region which is located between the FPV thymidine kinase (tk)gene and the 3'-open reading frame. Said intergenic region is enlarged to comprise one or more unique restriction sites, thereby allowing insertion of foreign DNA in such a way that the FPV tk-gene remains intact and codes for the entire thymidine kinase. New strong poxvirus promoters are presented and new FPV host virus strains carrying a vaccinia virus thymidine kinase gene and an E. coli lacZ gene as a novel non-essential site. The novel fowlpox virus host strains allow the use of any insertion plasmid carrying vaccinia virus tk-gene flanking regions.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.