Two-dimensional aggregation and fixation of protein by injection into a substrate solution having higher surface tension and specific gravity
US5670624A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 20, 1995 |
| Grant date | Sep 23, 1997 |
| Priority date | — |
| Expiry date | Sep 20, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/812
- WIPO fieldMicro-structural and nano-technology
- WIPO sectorChemistry
Abstract
Protein is two-dimensionally aggregated and fixed to produce a functional thin film. A denatured film of a first protein is formed on the surface of a substrate solution such as an aqueous sugar or salt solution. A solution of a second protein which may be the same as or different from the first protein is then injected below the surface of the substrate solution at a distance sufficient not to disturb the surface. The substrate solution has a higher surface tension and specific gravity than the second protein solution causing the second protein to float between the denatured protein film and the substrate solution surface to form the two-dimensionally aggregated and fixed protein. The denatured film of first protein may be formed by injection of a solution of the first protein below the surface of the substrate solution. The aggregated and fixed protein can be heated and then transferred onto a solid surface such as in the formation of a biosensor. A three-dimensional multilayer laminate of the two-dimensional aggregate may also be formed. In an alternative embodiment, a lipid monolayer is formed on the surface of the substrate solution, a protein solution is injected below the su…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.