Recombinant core streptavidin

US5672691A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Erhard Kopetzki · Penzberg, DE
• Rainer Rudolph · Waldshut-Tiengen, DE
• Adelbert Grossmann · Murnau am Staffelsee, DE

Key dates

• Filing date: May 4, 1995
• Grant date: Sep 30, 1997
• Expiry date: May 4, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/36
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention concerns a process for the isolation of recombinant core streptavidin in which host cells are transformed with a DNA coding for core streptavidin, the transformed host cells are cultured under suitable conditions, the DNA coding for core streptavidin is expressed and the recombinant core streptavidin is isolated from the host cells or the culture medium, wherein a DNA coding for core streptavidin is used which has PA1 (a) the nucleotide sequence shown in SEQ ID NO. 1 or PA1 (b) a nucleotide sequence corresponding to the nucleotide sequence (a) within the scope of the degeneracy of the genetic code.