Charge-coupled device imaging apparatus
US5672881A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 6, 1996 |
| Grant date | Sep 30, 1997 |
| Priority date | — |
| Expiry date | Mar 6, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/6456
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus for fluorescence of a biological sample includes a light illumination source, an ambient light-excluding optical chamber, a sample holder, a two-dimensional array charge coupled device ("CCD") sensor providing for on-chip signal integration of each pixel of the array, and an analog/digital converter for digitizing the CCD signal. Signal acquisition time from the array is varied so that the integrated signal from each pixel is below its saturation level. A darkfield value for each pixel is acquired and subtracted from the illuminated signal from that pixel so as to yield a darkfield corrected signal. The fluorescence value for each pixel can be compensated for variations in excitation energy reaching different sample areas. Image readout is through a single channel output.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.