Stem-loop and circular oligonucleotides and method of using
US5674683A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 21, 1995 |
| Grant date | Oct 7, 1997 |
| Priority date | — |
| Expiry date | Mar 21, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6839
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides stem-loop and circular oligonucleotides each with at least one Watson-Crick binding (WC) domain and at least one corresponding Hoogsteen binding (H) domain separated from each other by linker domains. Each WC domain has sufficient complementarity to bind to one strand of a defined nucleic acid target by Watson-Crick base pairing in an anti-parallel orientation. Each corresponding H domain is capable of binding to the WC domain by Hoogsteen base pairing in an anti-parallel manner. The present invention also provides methods of making and using these oligonucleotides as well as kits and pharmaceutical compositions containing these oligonucleotides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.