Two-phase optical assay
US5674699A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 22, 1994 |
| Grant date | Oct 7, 1997 |
| Priority date | — |
| Expiry date | Dec 22, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/723
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is provided for measuring an analyte in a sample comprising adding substantially transparent particles to a sample in solution or suspension, said particles having an affinity for said analyte; fractionating the particles from the solution or suspension to form a particle-rich fraction and a substantially particle-free fraction; optically reading the particle-rich fraction at a first and a second wavelength; optically reading the substantially particle-free fraction at at least the first wavelength; and correlating the readings through the particle-rich fraction and the substantially particle-free fraction of the sample, with similar measurements in a particle-containing "blank" to obtain a quantitative determination of the analyte originally present in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.